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Search life-sciences literature (Over 39 million articles, preprints and more)
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Polyethylene terephthalate (PET) and polylactic acid (PLA) are among the polymers used in the food industry. In this study, crude extracts of Dunaliella salina were used to treat the surface of 3D printed materials studied, aiming to provide them with an anti-adhesive property against Pseudomonas aeruginosa.The hydrophobicity of treated and untreated …
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Summary: Pseudomonas aeruginosa PAO1 possesses two distinct lipopolysaccharide (LPS) O-polysaccharide species, A- and B-band LPS, the relative expression of which appears to be under environmental control. In an attempt to identify the influence these LPS types have on surface characteristics and adhesion, we examined the surface hydrophobicity and surface charge of …
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f Pseudomonas aeruginosa 10,145, Staphylococcus epidermidis 9142 (biofilm-positive), and 12,228 (biofilm-negative) to two extended-wear silicone–hydrogel lenses (balafilcon A and lotrafilcon A), a daily wear silicone–hydrogel lens (galyfilcon A) and a conventional hydrogel (etafilcon A) were performed. To interpret the adhesion results, lens surface relative …
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The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin …
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Here, we use atomic force microscopy techniques to quantify the forces guiding the adhesion of Pseudomonas aeruginosa type IV pili to surfaces. Using chemical force microscopy and single-cell force spectroscopy, we show that pili strongly bind to hydrophobic surfaces in a time-dependent manner, while they weakly bind to hydrophilic surfaces. ...
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In an earlier study, Pseudomonas putida NCIB 9816-4, which was one of the most studied naphthalene-degrading bacteria, showed the preferred adhesion to the naphthalene-contaminated soil when it was in the exponential growth phase. The adhesion was found to take place through a hydrophobic interaction. We postulated that the surface …
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The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film …
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On the basis of their adhesion, a distinction could be made between three adhesive and three non-adhesive strains of P. aeruginosa, while bacterial motilities and zeta potentials were comparable for all six strains. However, water contact angles indicated that the adhesive strains were much more hydrophobic than the non-adhesive strains.
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The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two …
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The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH 7) under controlled flow.
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Objectives: The adhesion of colloidal probes of stainless steel, glass and cellulose to Pseudomonas fluorescens biofilms was examined using atomic force microscopy (AFM) to allow comparisons between surfaces to which biofilms might adhere. Results: Biofilm was grown on a stainless steel substrate and covered most of the surface after 96 h.
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In addition to SRB, Pseudomonas aeruginosa, a facultative aerobic bacterium, is another prominent erosive microorganism that has been extensively investigated in marine environments.It exhibits a preference for attaching to metals during biofilm formation, acting as a pioneer colonizer that facilitates the subsequent attachment of anaerobic bacteria [2], [30].
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Biotechnology Letters, 2016. Objectives: The adhesion of colloidal probes of stainless steel, glass and cellulose to Pseudomonas fluorescens biofilms was examined using atomic force microscopy (AFM) to allow comparisons between surfaces to which biofilms might adhere.
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A monoculture of Pseudomonas fluorescens was chosen as the fouling microorganism for the laboratory studies. Results indicate that the adhesive strength of the biofilm was affected by the conditions of operation, such as biofilm age, nutrient concentration, suspended cell concentration, pH, surface roughness of the substratum and fluid velocity.
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2.1. Hydroquinine Inhibits P. aeruginosa Growth through Decreased Expression Levels of Adhesion-Related Genes. To validate the expression of the adhesion-related genes in response to hydroquinine, we identified that at 1.25 mg/mL, hydroquinine reduces adhesion-related gene expression in P. aeruginosa ATCC 27853 (Figure 1).The quantitative reverse …
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We investigated the impact of calcium and magnesium ions on the deposition kinetics of flagellated and nonflagellated Pseudomonas aeruginosa onto an alginate conditioning film in a radial stagnation point flow system. The bacterial deposition/adhesion behavior was related to structural changes of th …
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Most bacteria carry a net negative surface charge, thereby promoting adhesion on positively charged materials (Gottenbos et al., 2001, Gottenbos et al., 2003, Dunne, 2002).Stenotrophomonas maltophilia is an exception to this rule, possessing an overall positive surface charge at physiological pH (Jucker et al., 1996).Bacterial cell wall polymers such as …
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Pseudomonas aeruginosa is a pioneer bacterium that can initiate biofilm formation in a seawater environment, causing biofouling and biocorrosion. Therefore, understanding the interactions that occur between this bacterium and a commonly used material, 304 stainless steel, in the marine environment is mandatory before attempting to identify methods that can …
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O'Toole GA, Kolter R (1998) Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol 30:295–304 Busalmen JP, de Sanchez SR …
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Bacteria experience fluid flow in natural environments and during host colonization. As a result, many bacterial species have evolved elaborate mechanisms to colonize environments with high shear forces (1 –6).For example, Escherichia coli uses the protein FimH to promote adhesion in the human urinary tract (7 –9).FimH is remarkable as it mediates strong …
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Therefore, we have tested these oligosaccharides for their inhibitory potential of pathogen adhesion in two different human epithelial cell lines. 2′-FL could inhibit adhesion of Campylobacter jejuni, enteropathogenic Escherichia coli, Salmonella enterica serovar fyris, and Pseudomonas aeruginosa to the intestinal human cell line Caco-2 ...
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The adhesion of Pseudomonas fluorescens (ATCC 700830) to perpendicularly polarized magnetic surfaces was recently discovered. The findings have found that the magnetic free surfaces from different magnetic polarities have different profound effects on the P. fluorescens bacterial adhesion to its surfaces. These phenomena can be explained by the …
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Purpose: The purpose of this study is to compare the adhesion capabilities of the most important etiologic agents of microbial ocular infection to the recently available silicone-hydrogel lenses with those to a conventional hydrogel lens. Methods: In vitro static adhesion assays of Pseudomonas aeruginosa 10,145, Staphylococcus epidermidis 9142 (biofilm-positive), and …
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Reducing bacterial adhesion on substrates is fundamental for various industries. In this work, new superhydrophobic surfaces are created by electrodeposition of hydrophobic polymers (PEDOT-F 4 or PEDOT-H 8) on stainless steel with controlled topographical features, especially at a nano-scale.Results show that anti-bioadhesive and anti-biofilm properties …
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Some properties of the adhesion-modifying factors of Pseudomonas fluorescens are described. Adhesin, which promotes the adhesion of P. fluorescens cells, is a hydrophobic compound of a protein nature with a molecular mass of more than 10 kDa located either at the cell surface or in the medium. Anti- …
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Transposon mutant strain 3G6 of Pseudomonas fluorescensATCC 17400, which was deficient in pyoverdine production, was found to produce another iron-chelating molecule, identified as 8-hydroxy-4-methoxy-quinaldic acid (designated quinolobactin), which is assumed to be the 75-kDa iron-repressed outer membrane protein (IROMP). Expand
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Abstract. Microbiologically contaminated water is a significant source of infections in humans and animals, with Pseudomonas aeruginosa (PSA) being particularly concerning due to its ability to thrive in water environments and its resistance to many disinfectants. Therefore, this study investigates the adhesion potential of PSA strains on various materials used in mineral …
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ABSTRACT The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin …
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The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film …
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Adhesion of Pseudomonas fluorescens (ATCC 17552) to Nonpolarized and Polarized Thin Films of Gold ...
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Adhesion of Pseudomonas putidaKT2442 Is Mediated by Surface Polymers at the Nano- and Microscale Caitlin H. Bell, Bhupinder S. Arora, and Terri A. Camesano* Department of Chemical Engineering Worcester Polytechnic Institute Worcester, MA 01609 ABSTRACT Pseudomonas putidaKT2442 is a bacterium with potential for use in bioremediation of ...
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The adhesion ability of P. aeruginosa also decreased to 23.0% at the level of 50.0 µM, indicating that there might not be a linear variation with further addition of the AHLs to promote such cell adhesion, and this agreed with the trend of biofilm formation discussed above.
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The chronic airway infections with Pseudomonas aeruginosa are the major co-morbidity in people with cystic fibrosis (CF). Within CF lungs, P. aeruginosa persists in the conducting airways together with human mucins as the most abundant structural component of its microenvironment. We investigated the adhesion of 41 serial CF airway P. aeruginosa …
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In this study, the adhesive exopolysaccharides of strains of Pseudomonas putida and P. fluorescens, both isolated from freshwater epilithic communities, were examined with regard to their chemical composition, biosynthesis, and their role in adhesion. Electron microscopy showed that both strains wer …
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The roles of lipopolysaccharides (LPS) and extracellular polymers (ECP) on the adhesion of Pseudomonas aeruginosa PAO1 (expresses the A-band and B-band of O antigen) and AK1401 (expresses the A-band but not the B-band) to silicon were investigated with atomic force microscopy (AFM) and related to biopolymer physical properties. Measurement of …
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Adhesive properties strains Pseudomonas aeruginosa allocated in 2003-2004 from clinical sicks, and also museum strains and strains allocated from agricultural animals and birds were studied. The interrelation with resistance to antibiotics these strains were found. The tendency of growth adhesion properties is marked at
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Overproduction of the Psl polysaccharide led to enhanced cell-surface and intercellular adhesion of P. aeruginosa. This translated into significant changes in the architecture of the biofilm. We propose that Psl has an important role in P. aeruginosa adhesion, which is critical for initiation and maintenance of the biofilm structure.
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